Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State

The mammalian ovary is a large source of oocytes organized into follicles at various stages folliculogenesis. However, only limited number them can be used for in vitro embryo production (IVEP), while most have yet to complete growth and development attain full meiotic embryonic developmental competence. While the primordial ovarian cortex has potential produce mature oocytes, it still an experimental stage. population early antral (EAFs), instead, may represent reserve close completing phase, which might more easily exploited could increase female gametes dedicated IVEP.Here we present culture strategies that been developed utilizing physiological parameters support specific needs distinct differentiation, order expand IVEP by maximizing attainment fertilizable oocytes. Furthermore, these systems provide powerful tools dissect molecular processes direct final differentiation oocyte.